Sample Answer:
Aim: To show the effect of temperature on the rate of enzyme activity
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Problem Statement
·
Variables
Manipulated variable:
Temperature
Responding variable: The rate
of enzyme / pepsin activity
// The time taken for albumen
suspension to turn clear
// The time taken for complete
hydrolysis of albumen /
substrate / protein / starch?
Controlled
variable: Concentration / volume of albumen (suspension) / pepsin (solution) //
pH
· Hypothesis
The higher the temperature
the longer / shorter the time taken for the complete hydrolysis of albumen /
starch. (Reject: substrate)
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·
List of materials and apparatus
*Albumen (suspension), *pepsin
(solution), distilled water and ice cubes.
*Water bath OR Bunsen burner, beaker and
thermometer, *Stopwatch, test tube, and measuring cylinder.
·
Experimental procedures
1. Label three test tubes, A, B
and C.
2. Fill each test tube with 5
ml of albumen suspension.
3. Add 5 ml pepsin into every
test tube.
4. Record the initial
observation on the albumen suspension.
5. Immerse the test tubes A in
water bath of 30°C, B in 40°C and C in 60°C.
6. Start the stopwatch.
7. Record the time taken for
the albumen suspension turns clear.
8. Calculate the rate of enzyme
activity using the following formula;
Rate of enzyme activity = 1/Time
9. Tabulate the data (in a
table).
10. Repeat the experiment /
steps 1-9 to get average readings.
(Accept if use other substrates
and enzymes.
Example: Materials; *starch,
*amylase solution and iodine solution.
Apparatus; white tiles, droppers )
·
Presentation of data
Temperature (°C)
|
The time taken for the
albumen suspension turns clear
(min)
|
Rate of
(enzyme) activity
(min-1)
|
30
|
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40
|
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50
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